Table 2. Primer sequences for target genes of interest.
| Target gene(abbreviation) | Primer sequence 5’– 3’ | Product size (base pairs) | Annealing temp (°C) | GenBank accession number | Reference |
|---|---|---|---|---|---|
| Toll-like receptor 2 (TLR2) | F: AGTGGCCAGAAAAGCTGAAA | 263 | 54.3 | NM_001005264 | [34] |
| R: ATCCAGTTGCTCCTTCGAGA | |||||
| Toll-like receptor 3 (TLR3) | F:GTCCAATTTCATTAAGGCCAAG | 129 | 54 | XM_540020.2 | This study |
| R:TGTCACTTGCTCAGTCTCCTTT | |||||
| Toll-like receptor 4 (TLR4) | F: CAGCATTCCAGTTTGAAGCA | 140 | 54.3 | AB080363 | This study |
| R: AAGACTTCGAGGCTGACCAA | |||||
| Toll-like receptor 9 (TLR9) | F: GCCTGGAGTACCTGCTCTTG | 128 | 55 | NM_001002998.1 | This study |
| R: GTTACGGGCATGGTCACAG | |||||
| Interleukin 17A (IL-17A) | F: CCGATCTACCTCACCTTGGA | 166 | 60 | NM_001165878.1 | [35] |
| R: TCGCAGAACCAGGATCTCTT | |||||
| Interleukin 22 (IL-22) | F: TCCAGCAGCCCTATATCACC | 254 | 60 | XM_538274.2 | [35] |
| R: TTGGCTTAGCTTGTTGCTGA | |||||
| Forkhead box P3 (FoxP3) | F: GGCTCCTGCTGTATCGTAGC | 179 | 55 | NM_001168461.1 | This study |
| R: CGCATGTTGTGGAATTTGAA |