Table 1.

Preparation of sickle RBC samples. SNP (80 μM) was added to sample 3 or sodium nitrite (10 μM) added to sample 4 and were incubated for 30 minutes at 37°C. Calcium chloride (2 mM) was then added to samples 2–4. The gray cells represent the samples that underwent hypoxia-reoxygenation cycling and when in the sample preparation the cycling was performed. Sample 1 remained a control and was not cycled.

		+80 μM SNP	+10 μM NO2−	+2 mM Ca2+
Sample	1 (n = 13)
	2 (n = 13)			X
	3 (n = 8)	X		X
	4 (n = 5)		X	X