Figure 9.

(A) MC3T3 cells were cultured in osteogenic medium (Osteo) or osteogenic medium supplemented with 300μM pVTK peptide (Osteo + Peptide) for 12 days and the media was collected during media changes every 2 days. Quantification of calcium levels in the media showed pVTK treatment resulted in significantly higher calcium levels at days 10 and 12 compared to osteogenic medium controls. [* p < 0.05 Osteo vs. Osteo + Peptide at a specific day, t-test, ^ p < 0.001 days 2, 4, 6, 8 vs. days 10 and 12 for osteo, 1 way ANOVA on Ranks, Student-Newman-Keuls post hoc test]; (B) MC3T3 cells were cultured in osteogenic medium or osteogenic medium supplemented with 300μM pVTK peptide, with or without 0.5 or 1mM of supplemental CaCl₂ for 12 days. Calcium levels (quantified from demineralized cell matrices) were significantly increased with increasing supplemental media calcium levels in osteogenic controls, but no increase in Ca (no mineralization) was seen in peptide treated groups (* p < 0.05 compared to groups indicated by bar, or * p < 0.05 Osteo vs. Osteo + Peptide at a specific calcium concentration; 1 way ANOVA on Ranks, Student-Newman-Keuls post hoc test within Osteo or Osteo + Peptide treatment across calcium concentration, t-test between Osteo vs. Osteo + Peptide for a specific calcium concentration)