Table 2.

3³ Full factorial designs for robustness study.

Run	Independent variables
Flow rate (mL/min)	Wavelength (nm)	pH of buffer
Run 1	0.8	248	2.8
Run 2	1.0	248	2.8
Run 3	1.2	248	2.8
Run 4	0.8	250	2.8
Run 5	1.0	250	2.8
Run 6	1.2	250	2.8
Run 7	0.8	252	2.8
Run 8	1.0	252	2.8
Run 9	1.2	252	2.8
Run 10	0.8	248	3.0
Run 11	1.0	248	3.0
Run 12	1.2	248	3.0
Run 13	0.8	250	3.0
Run 14	1.0	250	3.0
Run 15	1.2	250	3.0
Run 16	0.8	252	3.0
Run 17	1.0	252	3.0
Run 18	1.2	252	3.0
Run 19	0.8	248	3.2
Run 20	1.0	248	3.2
Run 21	1.2	248	3.2
Run 22	0.8	250	3.2
Run 23	1.0	250	3.2
Run 24	1.2	250	3.2
Run 25	0.8	252	3.2
Run 26	1.0	252	3.2
Run 27	1.2	252	3.2