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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Curr Protoc Cell Biol. 2015 Sep 1;68:24.5.1–24.5.31. doi: 10.1002/0471143030.cb2405s68

Figure 24.5.8.

Figure 24.5.8

Fluorescence recovery after photobleaching (FRAP) experiment was performed on supported lipid bilayers containing either 100 molecules/mm2 of CD80-YFP-CD28TM-ΔWΔT (A) or CD80-CFP-CD28TM-ΔT (B). The laser beam was focused to a diffraction-limited spot in the bilayer plane by filling the back aperture of the objective and was used to perform photobleaching in a small area. Images of the bilayers were collected before, immediately at, and after indicated times after photobleaching. Percentage recovery was calculated from background-subtracted images using the formula %recovery = [(intensity(t) – intensitypostbleach)/(intensityprebleach)] × 100, where the intensities are average fluorescence intensities from an area bounding the bleach spot. Data are average and standard deviation from six independent bleach spots.