Table 24.5.2. Transmembrane Domains that Would Not Have a GPI-Modification Sitea.
| Protein | Result | Domain length | Big-PI score |
|---|---|---|---|
| H-2Kb alpha chain | GPI | 23 | 2.92 |
| I-Ek beta chain | GPI | 22 | 6.42 |
| ICAM-1 | GPI | 24 | 3.4 |
| CD11c | GPI | 20 | 0.69 |
| MHCI related protein | GPI | 20 | 3.36 |
| B7H | GPI | 21 | 4.4 |
| CD4 | TM | 23 | −5.13 |
| LDL R. | GPI | 22 | 6.38 |
| MICA | GPI | 21 | 0.5 |
| MICB | GPI | 21 | 0.5 |
| CD45 | GPI | 21 | 6.16 |
| EPO R | GPI | 23 | 6.92 |
| Cadherin | GPI | 24 | 9.42 |
| CD19 | GPI | 24 | 4.78 |
| Insulin R | GPI | 21 | 2.94 |
| EGFR | GPI | 20 | 7.20 |
| NOTCH-1 | GPI | 21 | 4.25 |
| LFA-1 | GPI | 21 | 7.79 |
| NACR alpha4 TM4 (ACH receptor) | TM | 21 | −8.56 |
| CD2 | GPI | 26 | 4.53 |
| ICOS | GPI | 21 | 4.54 |
| Hematopoietic cell transducer | TM | 21 | −11.94 |
| CD28 | TM | 27 | −11.12 |
| CD8 | GPI | 21 | 3.31 |
| CD80 | GPI | 22 | −4.51 |
| Nicastrin | GPI | 21 | −0.33 |
a
Sequence comprising the extracellular domain of CD80, red fluorescent protein, and transmembrane domains from the proteins below were assembled as in step 4 of Basic Protocol 6 and were analyzed using the Big-PI predictor tool. We found transmembrane domains of some proteins that would not cause the proteins to become GPI-anchored when expressed. We chose the transmembrane domain of CD28 for use in generating the transmembrane protein expression constructs described herein.