Table 1. Primer sequences for RT-PCR.
Gene | Forward primer sequence (5'-3') | Reverse primer sequence (5'-3') |
---|---|---|
IL-4 | ATGTGTCATCCTGCTCTTCTTT | GACTGGGACTCATTCATGGTGC |
IL-5 | CAAAAAGAGAAGTGTGGCGAGG | TAGATAGGAGCAGGAAGCCCG |
IL-10 | GGACAACATACTGCTAACCGACTC | AAAATCACTCTTCACCTGCTCCAC |
IL-13 | GCAACGGCAGCATGGTATGGAG | TGGTATAGGGGAGGCTGGAGAC |
IFN-γ | CTCAAGTGGCATAGATGT | GAGATAATCTGGCTCTGCAGGATT |
COX-2 | CAGGAAGTTGGTGAGCTGGTATA | TTGTGTTCGCCTGTAGTGCATA |
TARC | CAGAACCGCATTGCCTCTG | TTGAAGGTGTCGGGCAGC |
β-actin | GCACCACACCTTCTACAATGAG | TTGGCATAGAGGTCTTTACGGA |