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. 2015 Sep;12(5):528–539. doi: 10.11909/j.issn.1671-5411.2015.05.013

Figure 7. Dexamethasone reduces endothelial NO production.

Figure 7.

Human EA.hy 926 endothelial cells were treated with either Dex (1, 10 or 100 nmol/L) or with the solvent control DMSO for 24 or 48 h and NO production bioassayed with RFL-6 reporter cells. EA.hy 926 cells were washed and subsequently stimulated with 10 µmol/L calcium ionophore A23187 for 2 min before the transfer of conditioned media to RFL-6 reporter cells. The cGMP content in the RFL-6 cells was measured by radioimmunoassay. Columns represent mean + SE, n = 3. **P < 0.01 compared with Ctl. Ctl: control; Dex: dexamethasone; NS: not significant.