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. 2015 Oct 15;6:852. doi: 10.3389/fpls.2015.00852

Figure 1.

Figure 1

SERK5 in Landsberg erecta (Ler) ecotype encodes a LRR-RLK with a normal RD motif. (A) Amino acid sequence alignment (position 401–450) of SERK1-5 in Col-0 ecotype and SERK5 in Ler ecotype. SERK5-Ler contains a normal RD motif. R is substituted by L at position 401 in SERK5-Col. (B) cDNA sequence alignment (position 101–150) of SERK5 in Ler, Col-0, C24, Lan-0, and Ws-2. SERK5 in Col-0 contains extra six nucleotides in alignment position 115–120. SERK5 in C24, Lan-0, and Ws-2 contains two-nucleotide (TA) deletion at alignment position 140–141. (C) Full protein sequence of SERK5 in Ler. Asterisk mark indicates the conserved lysine (K) in ATP binding domain. (D) Expression levels of BKK1 and SERK5 in Col-0 (left) and Landsberg erecta (Ler) (right) ecotype are shown in all plant tissue. Semi-quantitative RT-PCR was carried out with RNA isolated from the 4 weeks old Col-0 and Ler ecotype Arabidopsis grown in soil. The PCR amplification cycles for BKK1, SERK5 and quantitative control ACTIN2, are 30, 30, and 19, respectively. F, flowers; S, stems; L, rosette leaves; R, roots; SI, siliques.