Figure 4.
Overexpression of SERK5-Ler partially restores BR signaling in bri1-5. (A–D) 10-day-old seedlings grown on 1/2 MS medium were treated with or without 1 μM 24-epiBL for 3 h. Relative expression levels of CPD, DWF4, BR6ox2, and SAUR-AC1 was examined by qRT-PCR. Overexpression of SERK5-Ler partially recovers the sensitivity of BR-responsive genes in bri1-5 upon BL treatment. ACTIN2 was used as the reference gene. Values are means ± SE (n = 3). Experiments were repeated three times with similar results.