Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 15;6:852. doi: 10.3389/fpls.2015.00852

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Wu, Wu, Gao, Li, Yin, Lv, Zhao, Li and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

SERK5-Ler interacts with BRI1. (A) Overexpression of a kinase-inactive variant of SERK5-Ler (K301E) led to a dominant-negative phenotype resembling bri1-4. Mating-based split ubiquitin system (mbSUS) to show BRI1-SERK5-Ler interaction in vivo. Interaction was determined by the growth ability of mated yeast on selective medium (-AHLT) supplemented with 0, 75, 150, and 400 μM methionine. NubWT and NubG were used as positive and negative control, respectively. (B) BRI1 was cloned as a -Cub fusion, and SERK5-Ler, BKK1, and SERK5 were cloned as a Nub- fusion. (C) BRI1 was cloned as a Nub- fusion, and SERK5-Ler, BKK1, and SERK5 were cloned as a -Cub fusion.