Table 1.

Properties of archaeal DNA ligases.

Organism	Growth conditions	UniProt ID	PDB ID	Cofactor	T opt	Reference
Bacteriophage
Bacteriophage T4	Mesophile	P00970	—	ATP	37°C	[16, 37]
Crenarchaeota
Aeropyrum pernix	Hyperthermophile	Q9YD18	—	ATP, ADP	70°C	[38]
Desulfurolobus ambivalens	Acidophile/thermophile	Q02093	—		NR	[39]
Staphylothermus marinus	Hyperthermophile	A3DP49	—	ATP, ADP	75°C	[40]
Sulfolobus acidocaldarius	Acidophile/thermophile	Q4JAM1	—	ATP	85°C	[41]
Sulfolobus shibatae	Acidophile/thermophile	Q9P9K9	—	ATP, dATP	60–80°C	[42]
Sulfolobus solfataricus	Acidophile/thermophile	Q980T8	2HIX, 2HIV	ATP	NR	[28]
Sulfophobococcus zilligii	Hyperthermophile	D2CJS7	—	ATP, ADP, GTP	75°C	[22, 29]
Euryarchaeota
Archaeoglobus fulgidus	Hyperthermophile	O29632	3GDE	ATP	NR	[25]
Ferroplasma acidarmanus	Acidophile	S0AR65	—	ATP, dATP	30°C	[43]
Ferroplasma acidiphilum	Acidophile	Q2PCE4	—	ATP, NAD+	40°C	[41]
Haloferax volcanii (LigA)	Halophile	D4GYZ4	—	ATP	NR	[10]
Haloferax volcanii (LigN)	Halophile	D4GY98	—	NAD+	45°C	[10, 44, 45]
Methanothermobacter thermautotrophicus	Thermophile	Q50566	—	ATP, dATP	60°C	[46]
Picrophilus torridus	Acidophile	Q6L195	—	ATP, NAD+	65°C	[41]
Pyrococcus furiosus	Hyperthermophile	P56709	2CFM	ATP	55°C	[26, 27, 47]
Pyrococcus horikoshii	Hyperthermophile	O59288	—	ATP	70–90°C	[23]
Thermococcus sp. 1519	Thermophile	C0LJI8	3RR5	ATP	70°C	[31, 48, 49]
Thermococcus fumicolans	Hyperthermophile	Q9HH07	—	ATP, NAD+	65°C	[19]
Thermococcus kodakaraensis	Thermophile	Q9HHC4	—	ATP, NAD+	65°C	[20, 50]
Thermococcus onnurineus	Hyperthermophile	B6YTR4	—	ATP, NAD+	80°C	[21]
Thermococcus sibiricus	Hyperthermophile	C6A2U9	4EQ5	ATP	NR	[30]
Thermoplasma acidophilum	Acidophile	Q9HJ26	—	ATP, NAD+	65°C	[41]

T _opt: temperature optimum for the DNA ligase in vitro; NR: not reported.