Figure 1.

ILCP and ILC2P in the bone marrow heterogeneously express CXCR6. (a) Flow cytometry of lineage depleted (Lin: CD3ε, CD5, CD8, CD11c, CD19, TCRβ, TCRγδ, Ter119, Gr1, and NK1.1) adult bone marrow (BM) from Cxcr6 ^Gfp/+ Id2 ^Yfp/+ mice. Among Lin⁻ IL-7Rα ⁺ compartments are defined: CLP (filled gray, c-Kit^lo  Sca-1^−/lo Flt3⁺   α ₄ β ₇ ⁻), ILCP (red, c-Kit^lo  Sca-1^−/lo Flt3⁻   α ₄ β ₇ ⁺), and ILC2P (blue, c-Kit⁻  Sca-1^hi). Each compartment is analyzed for CXCR6-GFP and ID2-YFP expression (bottom histograms). (b) Cell culture of BM ILCP, selected as ID2⁺ CXCR6⁻ (upper panels) or ID2⁺ CXCR6⁺ (lower panels). Each population is sorted and cultured at 20 cells per well on OP9-DL4 stromal cells with IL-7, c-KitL, and Flt3L for 10 days and analyzed by flow cytometry. Indicated obtained progenies were analyzed for ID2-YFP, CXCR6-GFP, RORγt, and IL-7Rα expression. Results are representative of at least 3 experiments each ((a): n > 5), or 2 experiments ((b), at least 4 wells of each condition).