Figure 4.

Intestinal ILC compartments have heterogeneous expression of CXCR6 and egress markers. (a) Flow cytometry of Lin⁻ (Lin: CD3ε, CD5, CD8, CD11c, CD19, TCRβ, TCRγδ, Ter119, and Gr1) adult intestinal LP from Rag2 ^−/−  Cxcr6 ^Gfp/+ mice. ILC subsets are defined as cNK (filled gray, Lin⁻  NK1.1⁺  NKp46⁺ IL-7Rα ⁻), ILC1 (blue, Lin⁻ NK1.1⁺ NKp46⁺ IL-7Rα ⁻), ILC2 (red, Lin⁻  NK1.1⁻  Thy1.2^−/lo  Sca-1^hi  c-Kit⁻  ICOS^hi), ILC3 NCR⁺ (green, Lin⁻ NK1.1⁻  Thy1.2^hi  Sca-1^−/lo  c-Kit⁻ NKp46⁺), ILC3 NCR⁻ (orange, Lin⁻ NK1.1⁻  Thy1.2^hi  Sca-1^−/lo  c-Kit^med NKp46⁺ CD4⁻), and LTi-like (light blue, Lin⁻ NK1.1⁻  Thy1.2^hi  Sca-1^−/lo  c-Kit^med NKp46⁺ CD4⁺). Each compartment is analyzed for CXCR6-GFP expression. (b) Flow cytometry of Lin⁻ adult intestinal LP from Rag2 ^−/−  Cxcr6 ^Gfp/+ mice. ILC subsets are defined as ILC2 (red, Lin⁻ GATA-3⁺ RORγt⁻), ILC3 NCR⁺ (green, Lin⁻ GATA-3⁻ RORγt⁺ NKp46⁺ CD4⁻), ILC3 NCR⁻ (orange, Lin⁻ GATA-3⁻ RORγt⁺ NKp46⁻ CD4⁻), LTi-like (light blue, Lin⁻ GATA-3⁻ RORγt⁺ NKp46⁻ CD4⁺), and ILC1-cNK (blue, Lin⁻ GATA-3⁻ RORγt⁻ NKp46⁺ NK1.1⁺). (c) Histograms depicting levels of CD62L, CD69, CD44, CXCR6-GFP, and α ₄ β ₇ in Lin⁻ (filled gray) or ILC subsets (as defined in Figure 3(b)) in adult LP (upper panels) or mesenteric lymph nodes (mLN, lower panels) from Rag2 ^−/− Cxcr6 ^Gfp/+ mice. Results are representative of at least 3 experiments each ((a), (b), (c): n > 5).