Table 1.
Strains and plasmids used in this study.
| Strain/plasmid | Genotype | Reference/source |
|---|---|---|
| Strains | ||
| LG1458 | MG1655(DE3) ΔgudD ΔuxaC | Prather Lab |
| IB1863 | MG1655 ΔendA Δzwf ΔpfkB ΔsspB pfkA::114-pfkA(DAS+4) HK022::tetR-Ptet-sspB | (Brockman and Prather, 2015) |
| IB1379 | MG1655 ΔendA Δzwf ΔpfkB | (Brockman and Prather, 2015) |
| IB1486 | MG1655(DE3) ΔendA Δzwf ΔpfkB ΔsspB pfkA::114-pfkA(DAS+4) HK022::tetR-Ptet-sspB ΔgudD ΔuxaC | This study |
| IB2255 | MG1655(DE3) ΔendA Δzwf ΔpfkB ΔpfkA ΔgudD ΔuxaC | This study |
| IB2472 | MG1655(DE3) ΔendA Δzwf ΔpfkB ΔsspB pfkA::114-pfkA HK022::tetR-Ptet-sspB ΔgudD ΔuxaC | This study |
| JW2758-5 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔgudD785::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | (Baba et al., 2006) |
| JW3887-1 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔpfkA775::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | (Baba et al., 2006) |
| JW3603-2 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔuxaC782::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | (Baba et al., 2006) |
| IB1486-GA | IB1486 / pRSFD-IN-MI / pTrc-udh | This study |
| LG1458-GA | LG1458 / pRSFD-IN-MI / pTrc-udh | This study |
| IB2255-GA | IB2255 / pRSFD-IN-MI / pTrc-udh | This study |
| IB2472-GA | IB2472 / pRSFD-IN-MI / pTrc-udh | This study |
| Plasmids | ||
| pCP20 | Repa, AmpR, CmR, FLP recombinase expressed by λ pr under control of λ cI857 | CGSC #7629 |
| pRSFD-IN-MI | pRSR1030 ori, lacI, KanR, INO1 (S. cerevisiae) and MIOX (M. musculus) expressed under control of T7 promoter | (Moon et al., 2009) |
| pTrc-udh | pBR322 ori, lacI, AmpR, Udh (P. syringae) expressed under control of Trc promoter | (Yoon et al., 2009) |