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. 2015 Oct 14;15:692. doi: 10.1186/s12885-015-1717-8

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© Simone et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Measurement of bone resorbing activity of OCs stimulated by BC cells. The OCs capacity to produce erosive lacunae on calcium phosphate was measured after stimulation with CM from both untreated and Everolimus-treated BC cells. a Significant increases of the number of erosion pits (above) and percentage of resorbed area (below), were observed after incubating OCs with CM from both BC cells, whereas this effect was significantly suppressed by Everolimus (*p < 0.05). Results are means of three experiments. b Variation of erosive pit formation by von Kossa-staining of calcium phosphate slices seeded with OCs compared to culture conditions. CM from Everolimus-treated MCF-7 cells inhibited the bone resorbing activity (bottom, right) compared to the constitutive activity (above, right) and to untreated cells CM (bottom, left) (scale bar: 25 μm; 40x magnification)