Table 3.
Crystallographic and refinement statistics for mutant (A481T) Ago2-guide-target complexes
| Target RNA | t1-A | t1-G |
|---|---|---|
| PDB code | 4Z4H | 4Z4I |
| Space group | P1211 | P1211 |
| Unit cell dimensions | ||
| a (Å) | 55.69 | 55.60 |
| b (Å) | 116.60 | 116.60 |
| c (Å) | 70.10 | 69.62 |
| β (°) | 92.29 | 92.42 |
| Ago2 per ASU | 1 | 1 |
| Data collection | ||
| Wavelength (Å) | 0.99999 | 0.99999 |
| Resolution (Å) | 44.81–2.50 (2.61–2.50) | 44.69–2.80 (2.95–2.80) |
| Total reflections | 113332 | 85,923 |
| Unique reflections | 30,411 | 21,701 |
| Completeness (%) | 98.6 (98.3) | 99.2 (97.6) |
| Redundancy | 3.7 (3.8) | 4.0 (4.0) |
| I/σI | 9.2 (2.3) | 8.7 (2.4) |
| Rmerge | 12.7 (69.7) | 13.1 (60.0) |
| Rpim | 12.0 (65.0) | 12.0 (55.0) |
| Refinement | ||
| Resolution (Å) | 44.81–2.50 | 44.69–2.80 |
| R-free/R-factor | 21.13/17.21 | 23.32/19.18 |
| R.M.S. deviation | ||
| Bond distances (Å) | 0.008 | 0.005 |
| Bond angles (°) | 0.940 | 0.865 |
| Number of atoms | ||
| Non-hydrogen, protein | 6432 | 6412 |
| Non-hydrogen, RNA | 568 | 568 |
| Phenol | 28 | 21 |
| Isopropanol | 4 | 20 |
| Phosphate | 10 | 0 |
| Mg | 3 | 3 |
| Water | 135 | 23 |
| Ramachandran plot | ||
| Most favored regions | 95.19% | 94.67% |
| Additionally allowed | 4.68% | 5.33% |
| Generously allowed | 0.13% | 0.00% |