Figure 5. The effects of lactate on myometrial Ca²⁺ signalling .

A, simultaneous recording (i) of spontaneous force (top) and Ca²⁺ (Indo‐1 fluorescence, emission signals at 400 and 500 nm) showing the typical effects of lactate (5 mM) application on rat myometrium; inset (ii) is from the trace and expands and overlaps the force and Ca²⁺ records during the control period and during lactate application, to show the changes produced by lactate. B, comparison (i) of the effects of lactate using control spontaneous activity and after application of the L‐type Ca²⁺ agonist, Bay K8644 and (ii) comparing mean data and SEM for effects of lactate on contraction amplitude, duration, frequency and AUC, with and without Bay K8644. C, the effects of high K⁺ depolarization (40 mM) on force (top) and Ca²⁺ and the effects of lactate on both.