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The ROCK-to-MLCK ratio is negatively correlated with age. a Relative mRNA content for the target gene MLCK, normalized to reference genes GAPDH, ACTB and PGK1 plotted against the patients’ age (n = 21). There was no consistent correlation between MLCK and age. b Ratio of ROCK expression to MLCK expression, plotted gainst age (n = 21). Note that there was a significant negative correlation between the ROCK-to-MLCK ratio and age using all three reference genes
