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Forward and reverse primers of ROCK1, ROCK2, MLCK and GAPDH (from Molbiol). ACTB and PGK1 were detected with Qiagen Primer Assays
| Gene name | Forward primer | Reverse primer | PCR product |
|---|---|---|---|
| ROCK1 | AAAATTGTGTGAGGAGGACATGG | TTCATCCCAACATTCTTGGATCT | 279 bp |
| ROCK2 | GCAATGCGGTAAAAAGCGA | GGGAATCATGGTGTGACCAA | 217 bp |
| MLCK | GTCTTATGTTATCTTCCATTCTA | TATAATAAACTGTGGCAATACTG | 156 bp |
| GAPDH | AGAAGGCTGGGGCTCATTTG | AGGGGCCATCCACAGTCTTC | 285 bp |
