Table 3.
Pearson’s correlation coefficients between target gene (ROCK1, ROCK2 and MLCK, ROCK-to-MLCK ratios) and age using three different reference genes
| Target gene | Reference genes | ||
|---|---|---|---|
| GAPDH | ACTB | PGK1 | |
| ROCK1 ( n = 41) | −0.0043 | −0.1584 | −0.0732 |
| only male subjects (n = 33) | −0.0375 | −0.1577 | −0.1596 |
| ROCK2 ( n = 41) | −0.1436 | −0.0733 | 0.0812 |
| only male subjects (n = 33) | −0.0884 | −0.0404 | 0.0810 |
| MLCK ( n = 21) | 0.0201 | −0.4151* | 0.0593 |
| only male subjects (n = 18) | 0.2899 | −0.3549 | 0.0371 |
| ROCK1-to-MLCK ratio ( n = 21) | −0.2996 | −0.3325 | −0.3839* |
| only male subjects (n = 18) | −0.2802 | −0.4389* | −0.5364* |
| ROCK2-to-MLCK ratio ( n = 21) | −0.4757* | −0.4427* | −0.4298* |
| only male subjects (n = 18) | −0.4246* | −0.4694* | −0.5854** |
| ROCK-to-MLCK ratio ( n = 21) | −0.5297** | −0.4352* | −0.5181** |
| only male subjects (n = 18) | −0.4625* | −0.4981* | −0.6126** |
Correlation coefficients in bold indicate that statistical significance was reached using all reference genes (*P < 0.05, **P < 0.01; t-test)