Table 1.

Mode of acquisition and identification (see section 2.4 for details), structural detail, optimal sample amounts and their r², dynamic range, its quantification slopes and their r², LOQ for every lipid class (see Figs. S1 and S3 for additional information)

Lipid class	Mode of identification	Structural detail	Sample amount linear range (uL sample)/linearity (r2)	Dynamic range (μM)	Slope/Linearity (r2)
CER	Neg FTMS	Species	0.2–20/0.9997	0.05–250	0.9776/0.9980
CHOL	Pos FTMS	Molecular species	0.2–20/0.9935	20–5000	1.045/0.9969
DAG	Pos FTMS + MSMS	Molecular species	0.2–20/0.9968	0.5–500	0.9271/0.9995
HEXCER	Neg FTMS	Species	–b	1.5–150	0.9636/0.9953
LPA	Neg FTMS	Molecular species	–b	0.25–250	0.9874/0.9990
LPC, LPC O–	Neg FTMS	Molecular species	1.0–20/0.9860	2.5–250	1.017/0.9999
LPE, LPE O–	Neg FTMS	Molecular species	0.2–20/0.9925	0.15–150	0.9818/0.9980
LPI	Neg FTMS	Molecular species	1.0–20/0.9992	0.25–250	0.9794/0.9975
LPS	Neg FTMS	Molecular species	–b	1–250	0.9831/0.9977
PA	Neg FTMS + MSMS	Molecular species	–b	1–250	1.022/0.9967
PC	Neg FTMS + MSMS	Molecular species	0.2–2.0/0.9998	5–2500	1.101/0.9919
PC O–	Neg FTMS	Species	0.2–2.0/1.0000	5–2500	1.101/0.9919
PE	Neg FTMS + MSMS	Molecular species	0.2–20/0.9980	1–250	1.035/0.9981
PE O–	Neg FTMS	Species	0.2–20/0.9988	1–250	1.035/0.9981
PG	Neg FTMS + MSMS	Molecular species	–b	0.25–250	0.9467/0.9953
PI	Neg FTMS + MSMS	Molecular species	0.2–20/0.9965	0.25–250	0.9498/0.9997
PS	Neg FTMS + MSMS	Molecular species	–b	0.25–250	0.9460/0.9973
CE	Pos FTMS + MSMS	Molecular species	0.2–20/0.9976	2–500	0.9001/0.9969
SM	Neg FTMS	Species	0.2–20/0.9936	0.4–1000	0.9554/0.9970
TAG	Pos FTMS + MSMSa	Species	0.2–20/0.9965	1–250	1.025/0.9832

^aAlthough MSMS was used to increase the confidence of identification, we could not assign molecular species for the TAG (see text for details).

^bNot measurable in this reference sample.