Figure 6. Overexpression of AnNce102 suppresses myriocin-induced eisosome disassembly and causes mislocalization of PilA foci to septa.

Deconvoluted confocal images of WT (PilA-GFP) and alcA^p annnce102 (alcA^p AnNce102-mRFP) strains grown in the presence of (Ai) glucose, (Aii) ethanol, (Aiii) glucose and 20 μg/ml myriocin and (Avi) ethanol and 20 μg/ml myriocin. (B) The number of PilA foci every case was counted using the spot tool of the Imaris software and is shown as a graph. Representative confocal images of (C) PilA-GFP and (D) PilA-GFP alcA^p-AnNce102-mRFP strains, in the presence of ethanol as sole carbon source. Septa are visualized by calcofluor white staining and marked with white arrows. Insets show a higher magnification of the boxed area. (E) The number of PilA foci per septum was counted and is shown as a graph with averages presented as mean ± SEM, where n represents the number of cells examined from at least three independent experiments. Bars 5 μm.