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. 2015 Aug 21;156(11):4187–4199. doi: 10.1210/en.2015-1514

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Copyright © 2015 by the Endocrine Society

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Figure 5. — Expression of total ERβ and ERβ2 (hatched region) mRNA in the brain of aged (18-mo-old) female rats after varying periods of E2 deprivation. ERβ2 mRNA expression was measured in the (A) hypothalamus, (B) dorsal hippocampus, and (C) ventral hippocampus at 1, 4, 8, and 12 weeks after OVX (n = 6–10/age per treatment group). Animals were treated with either vehicle (safflower oil) or 2.5-μg E2 (dissolved in safflower oil). a, statistically significant difference (P < .05) in total ERβ; b, statistically significant difference (P < .05) in ERβ2 as determined by Tukey's honestly significant-difference test after two-way ANOVA. Data are expressed as mean ± SEM.