Figure 9.

Effects of varying periods of E2 deprivation on RNAPII activity in aged female rats. p-RNAPII and total RNAPII protein levels were measured by Western blotting in the (A and B) hypothalamus, (C and D) dorsal hippocampus, and (E and F) ventral hippocampus at 1, 4, 8, and 12 weeks after OVX (n = 6–10/age per treatment group). After E2 deprivation, animals were treated with either vehicle (safflower oil) or 2.5-μg E2 (dissolved in safflower oil). *, statistically significant difference (P < .05) between groups; #, statistically significant within groups as determined by Tukey's honestly significant-difference test after two-way ANOVA. Representative blot images combined from several gels used to quantify Western blottings by densitometry.