Figure 3.

A, TSH induction of PTX-3 mRNA in multiple strains of fibrocytes, GD-OFs, and H-OFs. Five strains of fibrocytes from patients with GD, 5 from healthy donors, and 3 strains each of GD-OFs and H-OFs were treated with nothing (control) or bTSH (5 mIU/mL) for 6 hours. Total RNA was extracted and subjected to RT-PCR for PTX-3 mRNA. B, Fibrocytes were treated with nothing (control) or bTSH 5 mIU/mL for 72 hours, monolayers were harvested, and RNA was extracted and subjected to RT-PCR for TSHR mRNA. C, Fibrocytes were treated with nothing, bTSH, or M22 (2 μg/mL) for 6 hours, and then RNA was subjected to RT-PCR for PTX-3 mRNA. D, Fibrocyte cultures were treated with nothing, bTSH, or Dex (10 nM) for 6 hours. RNA was subjected to RT-PCR for PTX-3 mRNA. Data are expressed as means ± SD of triplicates from 3 independent experiments. P values are as indicated.