Figure 4.

A, Anti-IGF-IR blocking antibodies, RV001 and 1H7, attenuate the induction by TSH of PTX-3 in fibrocytes. Confluent cultures were pretreated with nothing, RV001 (50 μg/mL) or 1H7 (10 μg/mL) for 16 hours and then incubated without or with bTSH (5 mIU/mL) for an additional 6 hours. Cellular RNA was subjected to RT-PCR for PTX-3 mRNA. B, Confluent fibrocyte cultures were treated with nothing, bTSH (5 mIU/mL), IGF-I (10 ng/mL), or the combination of bTSH and IGF-I for 6 hours. Cellular RNA was subjected to RT-PCR of PTX-3 mRNA. Data are expressed as mean ± SD of triplicate replicates.