Figure 1.

THs promote Akt phosphorylation in HUVECs. Before performing the experiment described below, HUVECs were incubated for 4 h at 37°C with RPMI 1640 medium containing 0.1% fatty acid–free BSA to avoid the effect of serum and THs. A, HUVECs were incubated for 10 min at 37°C with the indicated concentrations of T₃ or T₄ to measure Akt phosphorylation activity by Western blotting analysis. B, The relative amount of Akt phosphorylation was calculated from three independent experiment shown in panel A. C, HUVECs were incubated for the indicated times with T₃ (1nM) or T₄ (1nM) to measure Akt phosphorylation activity by Western blotting analysis. D, The relative amount of Akt phosphorylation was calculated from three independent experiments shown in C. Representative Western blots of three separate experiments are shown in panels A and C. Results are means ± SEM of three separate experiments in panels B and D. *, P < .05 vs 0. Zero is none in panel B, and 0 minutes (baseline) in panel D.