Figure 5.

Neurotransmitter-regulated SST secretion from primary gastric epithelial cultures. Mixed gastric epithelial cultures from the corpus and antrum were incubated for 2 hours in bath solution containing the stimulatory neurotransmitters CGRP, VIP, and bombesin (all 100 nmol/L) (A) or acetylcholine (Ach) (10 μmol/L) with or without IBMX or GIP (100 μmol/L or 100 nmol/L, respectively) (B). SST was measured in the supernatant fraction and is expressed relative to basal secretion measured in parallel on the same day (control). FC is fold change compared with control (indicated by the dashed line); n numbers for each condition are represented above each bar. In B, the effect of Ach on IBMX and GIP was assayed in different preparations. The control data accompanying both Ach on IBMX and Ach on GIP experiments have been combined. Error bars represent 1 SE, and significance is shown analyzed by ANOVA with a post hoc Dunnett or Bonferroni modification where appropriate; ***, P < .001; ns, not significant.