Table 2.
RNA Sequencing Analysis of Select Genes Expressed in Gastric D-Cells and Neighboring Gastric Epithelial Cells
| SST Positive (FPKM) | SST Negative (FPKM) | |
|---|---|---|
| Coexpressed hormones | ||
| Sst | 82 900 ± 12 200 | 242 ± 34 |
| Pyy | 5260 ± 963 | 17.2 ± 4.9 |
| Ppy | 114 ± 18 | 0.55 ± 0.20 |
| Iapp | 38.8 ± 6.1 | 0.68 ± 0.59 |
| Gip | 67.7 ± 18.3 | 0.24 ± 0.14 |
| Cck | 37.2 ± 10.1 | 0.52 ± 0.22 |
| Hormone receptors | ||
| Glp1r | 8.23 ± 1.35 | 11.7 ± 2.7 |
| Gipr | 1.96 ± 0.32 | 0.02 ± 0.02 |
| Cckar | 3.53 ± 0.41 | 23.5 ± 6.7 |
| Cckbr | 0.19 ± 0.09 | 1.84 ± 0.72 |
| Sstr1 | 1.93 ± 0.84 | 0.02 ± 0.02 |
| Sstr2 | 1.01 ± 0.17 | 1.81 ± 1.22 |
| Sstr3 | 2.99 ± 0.62 | 0 ± 0 |
| Sstr4 | 0 ± 0 | 0 ± 0 |
| Sstr5 | 0.02 ± 0.01 | 0.01 ± 0.01 |
| Neurotransmitter receptors | ||
| Vipr1 | 2.00 ± 1.47 | 0.07 ± 0.07 |
| Calcrl | 50.9 ± 10.2 | 0.76 ± 0.25 |
| Ramp1 | 22.2 ± 2.3 | 0.68 ± 0.08 |
| Galr1 | 1.62 ± 0.33 | 0.07 ± 0.05 |
| Nmbr (Bb1) | 0.07 ± 0.07 | 0 ± 0 |
| Grpr (Bb2) | 0.09 ± 0.07 | 0 ± 0 |
| Brs3 (Bb3) | 0 ± 0 | 0 ± 0 |
| Gria2 | 6.00 ± 1.07 | 0.02 ± 0.02 |
| Grik5 | 1.12 ± 0.38 | 0.07 ± 0.04 |
| Chrm1 | 0.12 ± 0.05 | 0.80 ± 0.13 |
| Chrm2 | 0.04 ± 0.03 | 0 ± 0 |
| Chrm3 | 0.43 ± 0.06 | 0.32 ± 0.12 |
| Chrm4 | 9.80 ± 2.01 | 0.03 ± 0.03 |
| Chrm5 | 0 ± 0 | 0.04 ± 0.04 |
| Hrh1 | 0 ± 0 | 0.01 ± 0.01 |
| Hrh2 | 0.47 ± 0.11 | 0.76 ± 0.23 |
| Hrh3 | 0.27 ± 0.10 | 0 ± 0 |
| Hrh4 | 0 ± 0 | 0 ± 0 |
| Gabbr1 | 11.8 ± 3.1 | 0.97 ± 0.43 |
| Gabra1 | 3.69 ± 0.44 | 0.03 ± 0.01 |
| Gabrb3 | 35.3 ± 3.2 | 0.48 ± 0.16 |
| Nutrient-derived factor receptors | ||
| Casr | 7.32 ± 1.69 | 0.03 ± 0.03 |
| Taar1 | 6.81 ± 0.85 | 0.04 ± 0.04 |
| Gpr92/Lpar5 | 2.85 ± 1.12 | 2.08 ± 1.03 |
| Gprc6a | 0.12 ± 0.06 | 0 ± 0 |
| Tas1r1 | 0.01 ± 0.01 | 0.01 ± 0.01 |
Data are averaged FPKM values for genes of interest. Data are representative of 4 samples each from 4 separate mice for SST positive and negative cells.