Table 1.
Antibody Table
| Protein Name | Antibody Name | Manufacturer, Catalog Number, or Name of Individual Provider | Species Raised (Monoclonal or Polyclonal) | Dilution Used |
|---|---|---|---|---|
| Mac-2 | Rabbit antimouse Mac 2 | Cedarlane, 119–12296 | Rabbit polyclonal | 1:100 (IHC) |
| CD3 | Rabbit antihuman CD3 | Abcam, ab16669 | Rabbit monoclonal | 1:60 (IHC) |
| FcϵR1α | Antimouse FcϵRI-α | eBioscience, 14-5898-82 | Armenian Hamster monoclonal | 1:50 (IHC) |
| FcϵR1α | Antirabbit FcϵRI-α | Upstate, 06–725 | Rabbit polyclonal | 1:1000 (IB) |
| Bcl-2 | Bcl-2 antibody | Cell Signaling Technology, 2876S | Rabbit polyclonal | 1:1000 (IB) |
| p-JNK | Phospho-SAPK/JNK (Thr183/Tyr185) (G9) mouse mAb | Cell Signaling Technology, 5131 | Mouse monoclonal | 1:1000 (IB) |
| JNK | SAPK/JNK antibody | Cell Signaling Technology, 9252S | Rabbit polyclonal | 1:500 (IB) |
| p-AKT | Phospho-Akt (Ser473) (D9E) XP rabbit mAb | Cell Signaling Technology, 4060S | Rabbit monoclonal | 1:1000 (IB) |
| AKT | Akt antibody | Cell Signaling Technology, 9272S | Rabbit polyclonal | 1:3000 (IB) |
| Glut4 | Glut4 antibody (C-20) | Santa Cruz Biotechnology, sc-1608 | Goat polyclonal | 1:400 (IB) |
| CEBPα | C/EBPα antibody (14 AA) | Santa Cruz Biotechnology, sc-61 | Rabbit polyclonal | 1:1000 (IB) |
| PPARγ | PPARγ antibody (H-100) | Santa Cruz Biotechnology, sc-7196 | Rabbit polyclonal | 1:1000 (IB) |
| UCP1 | UCP1 antibody (C-17) | Santa Cruz Biotechnology, sc-6528 | Goat polyclonal | 1:3000 (IB) |
| β-Actin | Monoclonal anti-β-actin | Sigma, A5441 | Mouse monoclonal | 1:2000 (IB) |
Abbreviations: IB, immunoblot; IHC, immunohistochemistry; mAb, monoclonal antibody; SAPK, stress-activated protein kinase. This table is a list of antibodies used for immunoblot analysis and immunostaining.