Table 1. Comparison of the characteristics of ShadowG, mEGFP, and sREACh.
| Protein | EC (M−1cm−1) | QY | Abs (nm) | Em (nm) | Relative brightness | Folding t1/2 (sec) | Oxidation t1/2 (min) | Förster distance with mEGFP (nm) |
|---|---|---|---|---|---|---|---|---|
| mEGFP | 58000 | 0.73 | 488 | 507 | 100.0 | — | — | — |
| 56000* | 0.60* | 488* | 507* | — | — | — | — | |
| sREACh | 115000 | 0.07 | 517 | 531 | 19.0 | 308 | 133 | 5.8 |
| ShadowG | 89000 | 0.005 | 486 | 510 | 1.1 | 37 | 76 | 4.7 |
The quantum efficiency (QY) of fluorescent proteins was obtained using fluorescein in 1 M NaOH (0.925) as a reference. EC: extinction coefficient, t1/2: half-time of fluorescence recovery, Abs: absorption maximum, Em: Emission maximum.
The ECs were measured by means of the concentration of the chromophore determined by the alkaline denaturation method34.
*Values obtained from another study35.