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. 2015 Oct 15;5:15037. doi: 10.1038/srep15037

Figure 5. Representative images of complex I and IV abundance in skeletal muscle sections from control and disease cases using quadruple immunofluorescence.

Figure 5

Fluorescent detection was used to visualise: COX-I - green, NDUFB8 - purple, porin - red and laminin – white. Representative images of respiratory-normal tissue (disease control, DC1) and single large-scale mtDNA deletion (P6), multiple mtDNA deletions (P11), m.3243A > G MT-TL1 mutation (P16), m.5690A > G MT-TN mutation (P17), novel MT-TP mutation (P18), m.10010T > C MT-TG mutation (P19), m14709T > C MT-TE mutation (P20), m.5543T > C MT-TW mutation (P21) and m.3243A > T MT-TL1 mutation (P22). Representative images of compound heterozygous LRPPRC mutations and m.4175G > A MT-ND1 mutation are shown in Fig. 1. DC1 is a paediatric case and thus has smaller fibre size than the other cases. Scale bars measure 50 μm.