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. 2015 Oct 15;5:15403. doi: 10.1038/srep15403

Figure 1. HIV p17 regulates NRs and transcriptional coregulators in HepG2 cells.

Figure 1

(A) Immunoblot of syndecan-2 (Syn-2), CXCR2, and Tubulin. (B) qRT-PCR of syn-2 and CXCR2. Data represent the mean of 4 experiments. Values are normalized relative to GAPDH mRNA and are expressed relative to those of untreated cells, which were arbitrarily set to 1. *P,0.05 versus not treated cells. (C,D) Microarray analysis of nuclear receptors and transcriptional coregulators in HepG2 cells stimulated with p17 (10 μg/ml). Out of 84 genes examined, nine genes were upregulated 1.5-fold or greater (LXRα, MED1, DDX5, TR2, TR3, RARα, ESR1, NCOA1, NRIP1) while six genes were down-regulated 1.5 -fold or greater (PPARγ, KAT5, BRD8, PSMC5, HDAC7, ARNT). (C) Clustergram and (D) Scatter plot. (E) Validation of microarray results by RT-PCR showing the relative mRNA expression of LXRα, MED1, ESR1, DDX5, PPARγ, BRD8 and KAT5. Values are normalized relative to GAPDH mRNA and are expressed relative to those of untreated cells, which were arbitrarily set to 1. *P,0.05 versus not treated cells.