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. 2015 Oct 15;5:15403. doi: 10.1038/srep15403

Figure 3. Seven days treatment of HepG2 with p17 increases intracellular content of 25-hidroxicholesterol and induces lipid accumulation.

Figure 3

Intracellular content of 25-H-cholesterol (A) and total cholesterol (B) was measured in HepG2 cells stimulated with escalating doses of p17 (from 2 to 10 μg/ml) for 7 days. (C) Observation of lipid accumulation in HepG2 cells treated with 10 μg/ml p17 for 7 days. Representative photomicrographs (40X) of normal or p17 treated cells. (D) Oil Red O quantification at 500 nm.