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. 2015 Oct 15;5:15287. doi: 10.1038/srep15287

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Copyright © 2015, Macmillan Publishers Limited

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(a) Swimming motility was investigated on soft agar plates (0.3% Bacto Agar, 1% tryptone, 0.5% NaCl) containing 1 mM IPTG and 100 μg/mL ampicillin. Assays were performed at 30 °C for 12 h. A representative image of at least three independent experimental sets is shown. (b) The diameter of the swimming circle was compared to that of the control strain harboring the pHMB1 vector. Results are presented as the average of at least three separate experiments and error bars represent standard deviation. Normalized motility of the control strain is indicated with a black bar, and strains displaying >1.5-fold changes with a dark grey bar.