Figure 6. Effects of sRNA overexpression on type I fimbriae phenotypes.

Formation of mannose-specific type I fimbriae was determined by the ability of each strain to agglutinate yeast cells. Strains were grown in LB containing 1 mM IPTG and 100 μg/mL ampicillin without shaking at 37^oC and mixed with the same volume of yeast suspension (0.5% w/v, PBS) in a 96-well titer plate. Crystal violet was added to enhance observation. The data are representative of at least three independent experiments. The ΔfimA strain was used as a type I fimbriae-deficient control. Strains that appeared type I fimbriae-deficient are indicated with circles.