Figure 7. Effects of sRNA overexpression on curli fimbriae phenotype.

Strains harboring the sRNA library were assayed on a Congo red agar plate (LB agar without NaCl containing 1 mM IPTG, 100 μg/mL ampicillin, 40 μg/mL Congo red, 20 μg/mL Coomassie blue) to evaluate expression of curli fimbriae. Overnight cultures were streaked on agar plates and grown at 28 °C for 48 h. Strains showing curli fimbriae deficiency are indicated with white circles.