Table 1. Effects of E. coli sRNAs on biofilm phenotypes upon overexpression.
| sRNA | Relative Biofilma |
Motilityb |
Fimbriae |
||
|---|---|---|---|---|---|
| Swimming | Swarming | Type Ic | Curlid | ||
| Vector | 100 ± 6% | — | — | — | — |
| ArcZ | 18 ± 4% | ↓ | ↓ | — | — |
| C0067 | 91 ± 17% | — | — | — | — |
| C0293 | 83 ± 10% | — | — | — | — |
| C0299 | 81 ± 14% | — | — | — | — |
| C0343 | 73 ± 7% | — | — | — | — |
| C0362 | 78 ± 16% | — | — | — | — |
| C0465 | 90 ± 17% | — | — | — | — |
| C0614 | 87 ± 15% | — | — | — | — |
| C0664 | 99 ± 17% | — | — | — | — |
| C0719 | 99 ± 15% | — | — | — | — |
| CsrB | 145 ± 13% | — | ↓ | — | ↑ |
| CsrC | 182 ± 24% | — | ↓ | — | ↑ |
| CyaR | 80 ± 16% | — | — | — | ↓ |
| DicF | 11 ± 5% | ↓ | ↓ | — | NDe |
| DsrA | 2 ± 1% | ↓ | ↓ | ↓ | — |
| EyeA | 100 ± 10% | — | — | — | — |
| Ffs | 90 ± 12% | — | — | — | — |
| FnrS | 165 ± 5% | — | — | — | — |
| GadY | 116 ± 13% | — | ↓ | — | — |
| GcvB | 150 ± 10% | — | ↓ | — | — |
| GlmY | 122 ± 18% | ↓ | — | — | — |
| GlmZ | 139 ± 10% | — | — | — | — |
| IS118 | 66 ± 10% | ↓ | ↓ | ↓ | ↓ |
| IS128 | 83 ± 12% | — | — | — | — |
| McaS | 53 ± 16% | — | ↑ | — | ↓ |
| IsrB | 100 ± 13% | — | — | — | — |
| IsrC | 97 ± 12% | — | — | — | — |
| IstR-1 | 105 ± 12% | — | — | — | — |
| IstR-2 | 105 ± 13% | — | — | — | — |
| MgrR | 120 ± 5% | — | — | — | — |
| MicA | 71 ± 6% | ↑ | ↓ | ↓ | — |
| MicC | 104 ± 11% | — | ↓ | — | — |
| MicF | 96 ± 10% | — | — | — | — |
| MicM | 12 ± 2% | — | — | ↓ | ↓ |
| OhsC | 107 ± 8% | — | — | — | — |
| OmrA | 44 ± 4% | ↓ | ↓ | — | ↓ |
| OmrB | 108 ± 3% | — | — | — | ↓ |
| OxyS | 107 ± 3% | ↓ | ↓ | — | ↓ |
| PsrD | 109 ± 9% | — | — | — | — |
| PsrO | 105 ± 9% | — | — | — | — |
| RdlA | 103 ± 8% | — | — | — | — |
| RdlB | 106 ± 6% | — | ↓ | — | ↓ |
| RdlC | 103 ± 2% | — | ↓ | — | — |
| RdlD | 105 ± 2% | — | — | — | — |
| RnpB | 114 ± 2% | — | — | — | — |
| RprA | 82 ± 3% | — | ↓ | — | ↓ |
| RseX | 174 ± 12% | — | ↓ | — | ↓ |
| RttR | 97 ± 9% | — | — | — | — |
| RybA | 112 ± 13% | — | — | — | — |
| RybB | 10 ± 2% | — | — | ↓ | — |
| RybD | 113 ± 10% | — | — | — | — |
| RydB | 114 ± 3% | — | — | — | — |
| RydC | 99 ± 5% | — | ↓ | — | ↓ |
| RyeA | 108 ± 4% | — | — | — | — |
| SdsR | 57 ± 11% | — | ↓ | — | — |
| RyeF | 119 ± 10% | — | ↓ | — | — |
| RyfA | 56 ± 22% | — | ↓ | — | ↓ |
| RyfB | 127 ± 2% | ↓ | ↓ | — | ND |
| RyfD | 66 ± 13% | — | ↓ | — | — |
| RyhB | 92 ± 11% | — | ↓ | — | ↓ |
| RyjA | 88 ± 10% | — | — | — | — |
| RyjB | 94 ± 15% | — | — | — | — |
| SgrS | 160 ± 18% | — | ↓ | — | ↓ |
| SibA | 99 ± 13% | — | — | — | — |
| SibB | 88 ± 15% | — | — | — | — |
| SibC | 86 ± 14% | — | — | — | — |
| SibD | 99 ± 11% | — | — | — | — |
| SibE | 104 ± 14% | — | — | — | — |
| SokA | 101 ± 14% | — | — | — | — |
| SokB | 107 ± 14% | — | — | — | — |
| SokC | 87 ± 14% | — | — | — | — |
| SokE | 96 ± 11% | — | — | — | — |
| SokX | 87 ± 7% | — | — | — | — |
| Spf | 110 ± 12% | — | — | — | — |
| SraA | 122 ± 13% | — | — | — | — |
| SroA | 96 ± 13% | — | — | — | — |
| SroC | 63 ± 6% | — | — | — | — |
| SroD | 87 ± 14% | — | — | — | — |
| SroE | 98 ± 10% | — | — | — | — |
| SroG | 115 ± 10% | — | — | — | — |
| SroH | 112 ± 16% | — | — | — | — |
| SsrA | 120 ± 8% | — | — | — | — |
| SsrS | 134 ± 9% | — | — | — | — |
| SymR | 98 ± 9% | — | — | — | — |
| T44 | 106 ± 7% | — | — | — | — |
| Tp2 | 109 ± 8% | — | — | — | — |
| Tpke11 | 120 ± 7% | — | — | — | — |
| Tpke70 | 119 ± 10% | — | — | — | — |
| pRNA | 118 ± 8% | — | — | — | — |
| Och1 | 109 ± 9% | — | — | — | — |
| Och2 | 92 ± 10% | — | — | — | — |
| Och3 | 107 ± 10% | — | — | — | — |
| Och4 | 99 ± 11% | — | — | — | — |
| Och5 | 229 ± 10% | ↓ | ↓ | — | ND |
| Och6 | 115 ± 10% | — | — | — | — |
| Och7 | 108 ± 7% | — | — | — | — |
| Och8 | 98 ± 6% | — | — | — | — |
| Och9 | 112 ± 10% | — | — | — | — |
| Och10 | 119 ± 2% | — | — | — | — |
aAverage and standard error values of relative biofilm formation were calculated in at least three independent experiments.
b↓, >1.5-fold repressed, compared to pHMB1 vector; –, similar motility to pHMB1 vector; ↑, <1.5-fold changed, compared to pHMB1 vector.
c↓, unable to agglutinate yeast cells; –, able to agglutinate yeast cells.
d↓, formation of white-pink colony on Congo red indicator plates; –, similar color to pHMB1 vector; ↑, formation of dark red colony on Congo red indicator plates.
eND; Not determined due to toxicity on growth.