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. 2015 Oct 15;5:15085. doi: 10.1038/srep15085

Figure 1. HMGB1 induces clusterin from tumor cells and is released by dying tumor cells.

Figure 1

(A) DU145 prostate tumor cells, incubated with recombinant human HMGB1 (rhHMGB1) for 24–48 h, were lysed and analyzed by western blot for presence of clusterin, and for β-actin for equal loading. (B) DU145 tumor cells were treated with docetaxel (DTX) for 1–4 days and their supernatants were evaluated for HMGB1 by ELISA. (C) DU145 tumor cells were treated with the indicated chemotherapeutic agents for 24 h and the supernatants analyzed for HMGB1.