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. 2015 Oct 15;5:15085. doi: 10.1038/srep15085

Figure 3. Down-regulation of clusterin expression after inhibition of NFκB-p65 phosphorylation.

Figure 3

(A) Recombinant HMGB1, added to DU145 tumor cells, enhanced NFκB-p65 phosphorylation within 1–5 min, as analyzed by western blot. (B,C) DU145 tumor cells, pretreated with medium, 20 μM PS1145 or 10 μM BAY11-7082 for 1 h, were cultured with HMGB1 for another 24 h. Cells were then lysed for analysis of clusterin mRNA expression by Q-PCR or clusterin protein expression by western blot. Analysis of phosphorylated NFκB-p65 was included to check for effectiveness of the inhibitors.