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. 2015 Oct 15;5:15085. doi: 10.1038/srep15085

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(A) DU145 tumor cells, untreated or pretreated with control IgG, anti-TLR4, anti-RAGE, or both antibodies for 30 min, were exposed to recombinant HMGB1 for 24 h. Then, DTX was added for another 48 h prior to staining with methylene blue for visualization of live tumor cells. (B) The same experiment was conducted with recombinant HMGB1 substituted for supernatants from DTX-treated tumor cells. (C) DU145 tumor cells pretreated with recombinant HMGB1 were transfected with antisense-scramble or antisense-clusterin for 24 h, and then cells were cultured in medium with or without DTX for another 48 h prior to staining with methylene blue.