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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Chem Res Toxicol. 2009 Mar 16;22(3):511–517. doi: 10.1021/tx800369y

Figure 2.

Figure 2

Typical chromatograms obtained from LC-ESI-MS/MS analysis of Acr-modified pSP189 DNA. Double-stranded pSP189 DNA modified with different concentrations of Acr were denatured, digested with micrococcal nuclease, phosphodiesterase II, and alkaline phosphatase; the Acr-dG adducts were purified by SPE, and analyzed. Acr-dG adducts (upper trace) and standards of purified α-OH-Acr-dG and γ-OH-Acr-dG adducts (lower trace) was shown in. Note: two (+/−) stereoisomers of α-OH-Acr-dG were well separated while two (+/−) stereoisomers of γ-OH-Acr-dG cochromatographed at the same position (28).