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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Chem Res Toxicol. 2009 Mar 16;22(3):511–517. doi: 10.1021/tx800369y

Figure 5.

Figure 5

Acr-modification induced mutational spectrum in the supF gene in pSP189 plasmids replicated in human lung fibroblasts CCL-202. Mutations induced by Acr-modifications are shown in the lower panel and spontaneous mutations are shown in the upper panel. Methods for transfection and supF gene mutation detection are described in Materials and Methods. The mutant plasmids were purified (90 mutant plasmids resulted from plasmid modified with 1 and 2.5 mM Acr and 18 mutants plasmids resulted from unmodified plasmids) and the supF gene in these plasmids was sequenced. The symbols denoted are as follows: X, single base deletions; # ^, double mutations (two mutations occurring in the same plasmid); *, multiple mutation (more than two mutations occurring in the same plasmid); +, single base insertion; —X— fragment deletion.