Table 2.

Types of Mutations in the supF Gene in Acrolein-Treated pSP189 Plasmids Replicated in Human Normal Lung Fibroblasts CCL-202.

	CCL-202
	Control	Treated*
Single base substition
G•C to T•A	6 (43%)	44 (53%)
G•C to A•T	7 (50%)	25 (30%)
G•C to C•G	1 (7%)	10 (12%)
A•T to T•A	0 (0%)	1 (1%)
A•T to C•G	0 (0%)	1 (1%)
A•T to G•C	0 (0%)	2 (2%)
Total	14	83
Base substition**
G•C to T•A	6 (43%)	45 (48%)
G•C to A•T	7 (50%)	27 (29%)
G•C to C•G	1 (7%)	17 (18%)
A•T to T•A	0 (0%)	1 (1%)
A•T to C•G	0 (0%)	1 (1%)
A•T to G•C	0 (0%)	2 (2%)
Total	14	93
Single base substition	14 (78%)	83 (92%)
Single base deletion	0 (0%)	1 (1%)
Fragment deletion	4 (22%)	2 (2%)
Multiple muation***	0 (0%)	3 (3%)
Single base insertion	0 (0%)	1 (1%)
Total	18	90

^*Plasmids were treated with 1 or 2.5 mM Acr.

^**Including single base substitution, and multiple mutations.

^***Two or more than two mutations occur in the same plasmid.