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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Mol Nutr Food Res. 2011 Jun 29;55(9):1291–1300. doi: 10.1002/mnfr.201100148

Figure 1.

Figure 1

Acrolein distribution in normal human lung fibroblasts (CCL202). Cultured CCL202 cells were treated with Acr (0–100 μM, 1 h), fixed, stained with anit-Acr-FDP-lysine (Nε-(3- formyl-3,4-dehydropiperidino)-lysine) antibody followed by goat anti-mouse FITC-conjugated secondary antibody, and then examined by microscopy. The method is the same as previously described [8]. Note: DAPI stained DNA, Acr-FDP stained Acr-lysine adducts and Mito-tracker stained mitochondria.