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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Mol Nutr Food Res. 2011 Jun 29;55(9):1291–1300. doi: 10.1002/mnfr.201100148

Figure 2.

Figure 2

Analysis of acrolein induced DNA adduct under pH 8 and pH 9.8 reaction conditions by 32P post-labeling, 2 dimensional thin layer chromatography (2D-TLC) (A) followed by high performance liquid chromatography (HPLC). The DNA adducts in the indicated spots that contain Acr-dG were extracted and further separated by HPLC. Acr-dG, and Cro-dG co-migrate in 2D-TLC (B). Acr-dG, Cro-dG and HNE-dG can be well separated by HPLC (C). The methods for Acr-DNA modification and adduct analysis are the same as previously described [9, 11].