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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Leukemia. 2015 Jul 3;30(1):163–172. doi: 10.1038/leu.2015.174

Figure 1. Analysis of BAFF and IL-10 production by splenic CD5+CD19+ B cells in WT and EμTCL1-Tg mice.

Figure 1

(A) Serum IL-10 levels for WT and Eμ-TCL1-Tg mice at 12-mo (n=7 per group) were measured by ELISA. (B) Murine splenic B10 cells were gated by flow cytometry as B220+ CD5+ CD19+ IL-10+ as indicated in representative dot plots. Percentages of gated IL-10-expressing cells are indicated in the upper right quadrant of each plot (C) Percentages of gated IL-10-expressing B cells in WT mice and EμTCL1-Tg mice at 12-mo of age (n=8 per group) (D) Splenic B cells taken from WT mice and EμTCL1-Tg mice at 12-mo of age were stimulated with LPS or CpG and assessed for intracellular IL-10 expression by flow cytometry (n=8 per group) and (E) IL-10 secretion by ELISA. (F) Serum BAFF levels for WT and Eμ-TCL1-Tg mice at 12-mo (n=7 per group) were measured by ELISA. (G) Correlation between serum levels of BAFF and IL-10 in EμTCL1-Tg mice at 12-mo of age (n=7). In A-F, horizontal bars indicate the mean. In A-G, data are representative of at least two independent experiments.