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. 2015 Jul 6;35(11):1433–1444. doi: 10.1038/onc.2015.208

Figure 1.

Figure 1

FOXM1 complexes with OTUB1 in MCF-7 and MCF-7EpiR cells. Co-immunoprecipitation (co-IP) was performed with an IgG antibody control, a FOXM1 (α or anti-FOXM1) or an OTUB1 (αOTUB1) antibody on lysates from MCF-7 and MCF-7EpiR cells; Inputs (1/20 of IP), and IP products with IgG and specific antibodies were resolved on western blot and probed for (a) FOXM1 and (b) OTUB1. FOXM1* represents a FOXM1 species associated with its SUMOylation. (c) MCF-7 and MCF-7EpiR cells were treated with epirubicin (1 μM) for 0, 6 and 24 h. Co-IP was performed with an IgG antibody control and a FOXM1 antibody (αFOXM1); Inputs (1/10 of IP), and IP products with IgG and a FOXM1 antibody (αFOXM1) were resolved on western blot and probed for OTUB1. (d) Lysates prepared from epirubicin-treated MCF-7 and MCF-7EpiR cells as in (c) were precipitated with an IgG antibody control, a FOXM1 (αFOXM1) and an OTUB1 (αOTUB1) antibody and probed for FOXM1 expression.