Table 1.

Primers and probes used for real-time RT-PCR amplification of A/Leningrad/134/17/57 (H2N2).

Primers/probesa	Gene	Nucleotides	Sequence 5′→3′
NSLenF150	NS	150–169	CGGTCTGAACATCGAAACAG
NSLenR230	NS	210–230	AGTGCCTCATCGGATTCTTCC
NSLenP197r	NS	173–197	FAM-TCCACTATCTGCTTTCCAACACGGG-BHQ1
MLenF427	M	427–445	GCCTTGGGCCTGGTATGTG
MLenR488	M	465–488	CTATGAGACCTATGCTGGGAGTCA
MlenP447	M	447–463	FAM-AACCTGTGAACAGATTG-BHQ1
NALenF946	NA	946–965	TATGTGTGCTCAGGGCTTGT
NALenR1041	NA	1021–1041	TGGATTCCCTCTCTCATTGTT
NALenP967	NA	967–986	FAM-GGCGACACACCCAGGAACGA-BHQ1
NPLenF743	NP	743–764	CAGGAAATGCTGAGATCGAAGA
NPLenR813	NP	789–813	AGCAACTGACCCTCTCAATATGAGT
NPLenP769	NP	769–785	FAM-ATCTTTCTGGCACGGTC-BHQ1
PALenF1222	PA	1222–1242	CAGAATGAGTTCAACAAGGCA
PALenR1301r	PA	1282–1301	GGAGCCACATCTTCTCCAAT
PALenP1243	PA	1243–1265	FAM-TGCGAGCTGACCGATTCAATCTG-BHQ1
PB2LenF976	PB2	976–995	GGCGGGTTCACATTTAAGAG
PB2LenR1053	PB2	1034–1053	TGTTTGAAGATTGCCCGTAA
PB2LenP1026r	PB2	1001–1026	FAM-TTCCTCTCTCTTGACTGATGATCCGC-BHQ1

^aF and R in the primer name indicate forward and reverse direction, respectively. P indicates probe, and r indicates probe in reverse direction.